Role of Cinchona Alkaloids in the Enantio- and Diastereoselective Synthesis of Axially Chiral Compounds

Conspectus Asymmetric synthesis using organic catalysts has evolved since it was first realized and defined. Nowadays, it can be considered a valid alternative to transition metal catalysis for synthesizing chiral molecules. According to the literature, the number of asymmetric organocatalytic processes associated with atropisomer synthesis has rapidly increased over the past 10 years because organocatalysis addresses the challenges posed by the most widespread strategies used for preparing axially chiral molecules with satisfactory results. These strategies, useful to prepare a wide range of C–C, C–heteroatom, and N–N atropisomers, vary from kinetic resolution to direct arylation, desymmetrization, and central-to-axial chirality conversion. In this field, our contribution focuses on determining novel methods for synthesizing atropisomers, during which, in most cases, the construction of one or more stereogenic centers other than the stereogenic axis occurred. To efficiently address this challenge, we exploited the ability of catalysts based on a cinchona alkaloid scaffold to realize enantioselective organic transformations. Desymmetrization of N-(2-tert-butylphenyl) maleimides was one of the first strategies that we pursued for preparing C–N atropisomers. The main principle is based on the presence of a rotationally hindered C–N single bond owing to the presence of a large tert-butyl group. Following the peculiar reactivity of this type of substrate as a powerful electrophile and dienophile, we realized several transformations. First, we investigated the vinylogous Michael addition of 3-substituted cyclohexenones, where a stereogenic axis and two contiguous stereocenters were concomitantly and remotely formed and stereocontrolled using a primary amine catalyst. Subsequently, we realized desymmetrization via an organocatalytic Diels–Alder reaction of activated unsaturated ketones that enabled highly atropselective transformation with efficient diastereoselectivity, thereby simultaneously controlling four stereogenic elements. Employing chiral organic bases allowed us to realize efficient desymmetrizations using carbon nucleophiles, such as 1,3-dicarbonyl compounds, cyanoacetates, and oxindoles. These reactions, performed with different types of catalysts, highlighted the versatility of organocatalysis as a powerful strategy for atropselective desymmetrization of pro-axially chiral maleimides. Hereafter, we studied the Friedel–Crafts alkylation of naphthols with indenones, a powerful method for enantioselective synthesis of conformationally restricted diastereoisomeric indanones. We realized the first axially chiral selective Knoevenagel condensation using cinchona alkaloid primary amine as the catalyst. This reaction provided a powerful method to access enantioenriched olefins containing the oxindole core. Subsequently, we initiated an intense program for the computational investigation of the reaction mechanism of our atropselective processes. An understanding of the catalytic activity for vinylogous atropselective desymmetrization as well as of the role played by the acidic cocatalyst used for the experimental work was achieved. Recently, we have garnered interest in the novel frontiers of atropselective synthesis. As observed in recent publications, there is considerable interest in the development of methods for preparing N–N atropisomers, an emerging topic in the field of atropselective synthesis. We focused on the synthesis of hydrazide atropisomers by developing a one-pot sequential catalysis protocol based on two sequential organocatalytic reactions that provided high stereocontrol of two contiguous stereogenic elements.


INTRODUCTION
Asymmetric synthesis is an excellent method to selectively prepare chiral scaffolds. The high demand for methods to synthesize chiral molecules is driven by the fact that chirality is a fundamental property of bioactive natural products, drugs, and catalysts. Molecules that possess stereogenic carbon centers play a vital role in this field. However, in the past 15 years, molecules characterized by a form of chirality originating from restricted rotations along chemical single bonds, i.e., atropisomers, attracted several research groups owing to their innate properties; these molecules act as efficient ligands for asymmetric synthesis catalyzed by transition metals. 4 Famous ligands, such as BINAM, BINOL, or BINAP, represent core structures at the bases of complicated architectures designed and perfected over the years to improve the efficiency of enantioselective catalytic processes. However, atropisomers are not limited to ligands and catalysts. Owing to the abundant availability of natural compounds that display axial chirality, 5 several researchers have focused on preparing atropisomers as target compounds. Currently, enantioselective synthesis of atropisomers can be considered a mature field in the asymmetric synthesis scenario. The effectiveness of atroposelective transformations depends on two requirements to be met at the same time: (1) the rotational stability of the reaction product, which is, in most cases, the result of large steric hindrance around the axis of chirality, and (2) control over stereochemistry exerted by the catalyst when sterically hindered substrates are used. The first requirement is an intrinsic aspect of atropselective transformations because without steric hindrance, rotational stability cannot be achieved. However, this aspect poses a challenge for new atropisomers and can be satisfied by deploying sterically encumbered reaction partners. The second requirement represents the main core of any asymmetric synthesis; however, owing to the twisted conformation of atropisomers, appropriately designed multifunctional catalysts that can simultaneously coordinate reaction partners, giving a twisted imprint to the stereodetermining transition state reaction, are required. Chiral phosphoric acids, short peptides, and cinchona alkaloids are good promoters of atropselective transformations.
Effective catalytic tactics and strategies were reported with regard to the use of organo-based or transition metal based catalysts. 6 Further, C(sp 2 )−C(sp 2 ) atropisomers, which have been the base for the first studies on atropisomerism, are considered important. Among diffuse atropisomers, biaryls are characterized by high rotational energy barriers with values >30 kcal/mol, which endow them with high stereochemical stability. 7 Moreover, a rapid escalation of different classes of atropisomeric compounds has been observed over the past 10 years. Accordingly, C−S, C−O, and specifically C−N atropisomers have been investigated in detail. 8 Particularly, C−N atropisomers represent an important category of stereoisomers and are often found in biologically active natural products, for which the hindered C−N single bond is the key stereogenic element that underlies their biological properties. Most strategies employed to accomplish the stereoselective preparation of atropisomers are based on dynamic kinetic resolution and desymmetrization. However, in recent years, novel reactions have been reported as direct synthesis strategies, in which the stereogenic axis is generated in a single chemical operation. Currently, coupling reactions, chirality conversion, arylation, and arene-forming reactions are considered to be the most versatile synthesis methods. 9 Herein, we report the results of asymmetric synthesis of atropisomers using organic catalysts based on the cinchona alkaloid scaffolds, which can help exert the tridimensional arrangement of the chiral axis. We highlight how these chiral organocatalysts can control the stereogenic axis and concomitant presence of more than one stereogenic center exploiting their ability to interact with various substrates through different activation modes ranging from covalent enamine/ iminium ion catalysis to counterion-directed catalysis through hydrogen bonding and π-stacking interactions. The versatility expressed and possibility to be easily functionalized make these commercially available catalysts a mild alternative to metalbased catalysts.

DESYMMETRIZATION STRATEGY FOR SYNTHESIZING C−N ATROPISOMERS
Among organocatalytic strategies that allow one to build a stereogenic axis, desymmetrization reactions occur on prochiral or meso-compounds, which possess a rotationally constrained single bond. 10 Therefore, the stereoselective symmetry break is accomplished if the chiral catalyst can distinguish between the two atropotopic faces of the substrate, which originate from the plane of symmetry containing the axis. The first organocatalytic reactions demonstrated desymmetrization of biaryls and were performed using different strategies based on electrophilic and nucleophilic aromatic substitution reactions. In 2013, Akiyama et al. reported the selective bromination of tetrasubstituted biaryls catalyzed by chiral phosphoric acid (Scheme 1a). 11 The reaction was performed on 2′-(alkoxymethyl)-[1,1′-biphenyl]-2,6-diol 1, which was carefully designed, in which two hydroxy groups established intramolecular and intermolecular hydrogen bonds, which are beneficial for the reaction in terms of reactivity and stereoselectivity. Catalyst 2 promoted bromination was realized through the concomitant activation of electrophilic Nbromosuccinimide and nucleophilic aromatic diols in a highly organized cyclic transition state, thus ensuring excellent enantiocontrol.
In 2014, Smith developed a technique for desymmetrization of dichloropyrimidines 5 based on an organocatalytic nucleophilic aromatic substitution mechanism (Scheme 1b). 12 The reaction was performed using a phase transfer catalyst that promoted addition of thiophenol anions to the activated pyrimidine core. N-Benzylquininium chloride 6 efficiently desymmetrized substituted dichloropyrimidines in a highly

Scheme 2. Behavior of N-Arylmaleimide in Desymmetrization Reactions
Accounts of Chemical Research pubs.acs.org/accounts Article selective manner. In the aforementioned cases, kinetic resolution occurring during the reaction increased the enantiomeric excess, thereby releasing a negligible amount of the achiral product and almost enantiopure axially chiral biaryl. The first case of organocatalytic desymmetrization via vinylogous Michael addition to N-(2-tert-butylphenyl)maleimides, which led to the formation of C−N atropisomeric succinimides, was reported by us in 2014. 1 Maleimides have been widely used in different organic reactions owing to the strong electrophilicity of their double bond. 13 Additionally, the corresponding succinimide is an important moiety found in pharmaceutical and biological compounds. 14 Before our organocatalytic approach, preliminary studies conducted by Curran et al. on alkyl radical addition to N-arylmaleimides were fundamental to comprehend the interesting features of these compounds. 15 Particularly, a bulky substituent placed at the ortho position of the phenyl ring demonstrates two main effects: (1) It limits the free rotation around the N−C bond, thus (2) It shields one side of the maleimide from a nucleophilic attack via steric hindrance (Scheme 2). These aforementioned effects were best expressed in the Giese reaction of N-(2-tert-butylphenyl)maleimide 9 that directed the tert-butyl radical to the face not shielded by the ortho substituent, thus forming a racemic mixture of one prevailing diastereoisomer (Scheme 3).
We performed a desymmetrization reaction using an organocatalyst, which can recognize the atropotopic faces of maleimide and regioselectively direct the nucleophilic attack on one of the two accessible vinylic carbon atoms. For this purpose, we assumed that the stereoselective vinylogous Michael addition between 3-substituted cyclohexenones 12 and N-(2-tertbutylphenyl)maleimides 9 could be promoted using a primary amine through formation of a chiral dienamine. We screened different primary amines paired with two equivalents of acid cocatalyst to determine the best combination. Accordingly, we found that 9-amino-(9-deoxy)-epi-quinine 13 in combination with N-Boc-L-phenylglycine 14 afforded the best catalytic salt that can control simultaneous construction of three stereogenic elements in a highly stereoselective way (Scheme 4).
Nuclear Overhauser Effect (NOE) experiments confirmed that the nucleophile always approaches the maleimide through the face, which is not hindered by the tert-butyl group, whereas X-rays on the crystals assigned R,R,S a , i.e., the absolute configuration, to the major diastereoisomer 15a. Surprisingly, we noted only traces of the product when the acid was not employed; moreover, there seemed to be no correlation between the chirality of the cocatalyst and stereoselectivity of the reaction. The results obtained indicated a good tolerance of the system toward maleimides bearing substituents in 4, but bulky substituents in 5, similar to tert-butyl, suppressed the reactivity 15a−d (Scheme 5).
Enones with different substituents on the double bond were less reactive but indicated a good level of conversion when treated with additional electrophilic maleimides 15e−g. Finally, with regard to the γ,γ-disubstituted enones, we recorded two opposite behaviors: non-prochiral γ,γ-disubstituted enones afforded product 15h with remarkable yields and enantiomeric excesses; however, when the substituents were different, the conversion considerably decreased (15i).
Further experiments indicated that the catalyst itself promoted epimerization at the exocyclic stereocenter, which affected the outcome of the diastereomeric ratio. Particularly, 13 could still condense on one of the enantioenriched products 15a, causing loss of the chiral information of the γ-carbon via dienamine−iminium ion equilibrium (Scheme 6). Therefore, the final diastereomeric ratio (d.r.) observed reflects the thermodynamic stability of two diastereoisomers, i.e., 15a and epi-15a, and it is not influenced by the catalyst.
Further, we were interested in elucidating the mechanism of vinylogous Michael addition. Instead of depending only on the experimental results collected so far, to get a better insight into the nature of activation and the stereoselection of the system, we investigated the geometry of the transition state (TS) through Scheme 6. Catalytic Cycle and Erosion of Diasteroselectivity due to Epimerization

Scheme 7. Geometry and Stability of Dienamines
DFT computational studies. 16 We selected the reaction between 3-ethylcyclohex-2-en-1-one 12 and N-(2-tert-butylphenyl)maleimide 9 as a model, which was catalyzed by 20 mol % of 13 and 40 mol % of 14 (Scheme 4). Before making any further assumption on the TS, we considered that the catalyst (treated as isopropylamine) could form two reactive dienamines, which possess a different configuration of the double bond (E or Z), and the calculations demonstrated that these two reactive dienamines are both formed during the reaction (Scheme 7).
In the context of the geometry of the TS, we faced the problem of disposing two molecules of the acid cocatalysts. We supposed that while N-Boc-phenylglycine protonated the

Scheme 9. Computed TSs for Nonepimerizable Substrates
Accounts of Chemical Research pubs.acs.org/accounts Article quinuclidine ring, the other N-Boc-phenylglycine could bridge reaction partners, thus cyclically activating the system. This model was proposed for four possible approaches between each dienamine isomer and maleimide, considering the two observed diastereoisomers (Scheme 8a). Further calculations on intermolecular distances allowed us to exclude half of these TSs. Finally, the remaining four TSs (TS1, TS3, TS5, and TS7) were processed using Autodock software, in which each molecule of the cocatalyst was treated as a feasible ligand, which must adjust its position to best fit into the pocket of the rigid receptor formed during these transition states. This ultimate refining calculation allowed us to measure the definitive energies for all the TSs (Scheme 8b). TS5 and TS7 were not considered over TS1 and TS3 because the quinoline ring pointed toward the cocatalyst and caused more steric clashes that interrupt the bridging interaction. The computational study confirmed the preference of the catalyst to direct Michael addition toward the pro-S a carbon of maleimide. A prediction of the experimental diastereomeric ratio based on the energy difference between the TS1 and TS3 results was incorrect, as the epimerization process covered the effective diastereoisomeric ratio resulting from the simple nucleophilic attack. When the same computational approach was repeated with a non-epimerizable substrate (16), the calculated dr agreed well with the experimental results (Scheme 9).
Thereafter, we speculated whether a notable level of stereoselection in desymmetrization of N-(2-tert-butylphenyl) maleimides could be achieved with other types of nucleophiles. Inspired by the study conducted by Melchiorre et al., 17 we explored the reactivity of 1,3-dicarbonyls and cyanoesters using pyrimidine-bridged cinchona alkaloid catalyst 22 (Scheme 10). 18 We found that even in this case, the cinchona alkaloids represented a valid tool in recognizing the atropotopic faces of pro-axially chiral maleimides. In the presence of 1,3-diketones and β-ketolactones, a high level of conversion and stereoselection were achieved using 22, which directed the Si face of the nucleophile toward the pro-S a carbon of the maleimide from its accessible face, thus providing a single diastereoisomer with the S,R,S a absolute configuration (Scheme 11). This system tolerated electron-poor and electron-rich maleimides as well as those bearing a highly sterically demanding tert-butyl group in position 5 of the phenyl group. In most cases, the catalyst controlled the concomitant formation of three stereogenic elements except in some cases, in which there was a critical Moreover, we explored the behavior of 22 in promoting Michael addition of α-cyano esters, again underlining the high adaptability of catalyst 22 toward different nucleophiles (Scheme 12). Indeed, 22 still ensures good enantiocontrol but with reactants bearing small substituents. The final product was obtained with the R,S,R a configuration, which is the opposite with respect to that observed for 1,3-dicarbonyls (21a−e).
In the context of the reaction mechanism, we hypothesized that the stereochemical outcomes depend on secondary interactions, such as π-stacking, provided by the quinoline group on one side of catalyst 22 and the hydrogen bond provided by the quinuclidine core on the other side. These two units generate a chiral pocket that helps create a rigid closed TS of the reaction. When we used cinchona alkaloids with free OH, a considerably low enantiomeric excess was obtained, confirming the fundamental role of the dimeric nature of 22 to reach high stereocontrol. Therefore, we envisaged a TS, in which the catalyst induces π-stacking interaction between the quinoline and aromatic ring of the maleimide, thus exposing the R a prochiral carbon of maleimide to the nucleophile activated by the quinuclidine ring ( Figure 1). This could be the reason for a decrease in stereoselection caused by using aromatic substituted 1,3-diketones or β-ketolactones: the aromatic moiety can interfere with π-stacking between the maleimide and quinoline moiety of the catalyst.
Following the atropselective organocatalytic Michael additions to maleimides, we considered oxindoles as possible nucleophiles, as they can afford chiral structures pivotal for pharmaceutical applications. 19 In 2012, Jiang studied asymmetric Michael addition between oxindoles and arylmaleimides (Scheme 13), 20 and along with this study, several other cases had been reported; however, atropselective variants are yet to be explored.
The first catalyst investigation showed that thiourea-functionalized cinchona alkaloid can exhibit promising results in terms of yield and stereoselectivity, thereby directing the attention toward bifunctional catalysts. The squaramide-functionalized 9-amino-(9-deoxy)-epi-cinchonidine 28 turned out to be the best selection for activating oxindole through deprotonation and Scheme 12. Scope of the Reaction for α-Cyanoesters   1). This implies that nucleophile activation is controlled by the tertiary nitrogen, whereas the squaramide with concomitant activation and coordination on maleimide helps control the geometry of the approach, thereby creating a compact TS and transferring the chiral information to the product. Encouraged by the excellent results obtained from desymmetrization of maleimides, we decided to explore this concept via the Diels−Alder (DA) reaction. 22 The first base-catalyzed asymmetric DA reaction was reported by Kagan, 23 in which Nmethylmaleimide reacted with anthrone in the presence of a catalytic amount of quinidine to afford the optically active product with excellent yields (Scheme 15a). The primary aminecatalyzed organocascade reaction between enones and maleimides was reported in 2009 by Melchiorre, 24 through which formal DA cycloadducts were obtained with great enantioselectivity (Scheme 15b). Therefore, we envisioned whether an atropselective version can be achieved under the influence of a primary amine organocatalyst, and in 2015, we realized the formal DA cycloaddition of α,β-unsaturated enamines to N-(2-tertbutylphenyl)maleimide, through which four stereogenic elements were simultaneously and selectively formed (Scheme 16). 25 The intention was to use a chiral primary amine catalyst to activate α,β-unsaturated ketones and form a stereodefined enamine intermediate that will serve as diene in atroposelective cycloaddition. Amino quinine 13 was revealed to be the best catalyst in combination with two equivalents of 2-hydroxybenzoic acid 39, forming the product 40 as a single diastereoisomer with high yield and stereocontrol. The reaction proceeds based on the endo rule during the cycloaddition step, as typical for dienophiles possessing suitable conjugating substituents. The catalyst drives the diene approach on the opposite side from the tert-butyl group and interacts with the atropotopic Si face of maleimide, suggesting a favored TS in which stabilizing hydrogen bonding occurs between the carbonyl of the electrophile and quaternary nitrogen of the quinuclidine. Again, 9-amino-(9-deoxy)-epi-quinine 13 could remotely control the configuration of the stereogenic axis and that of three contiguous stereocenters. Various unsaturated ketones bearing different substituents on the aromatic ring reacted smoothly; substituents in position 4 of the maleimide were tolerated, whereas those in position 5 sterically hampered the approach of enamine (40a−f). Catalyst 13 could not realize the DA reaction when alkyl substituents were used, and this acted as a limitation to the reaction scope. Generally, for cinchona organocatalysts, access to both enantiomers of a product is easily realized. Accordingly, herein, the pseudoenantiomer ent-13 catalyst produced ent-40c with high stereoselectivity.

SYNTHESIS OF ALKYLIDENE CYCLOHEXANONES VIA AXIALLY ENANTIOSELECTIVE KNOEVENAGEL CONDENSATION
In addition to atropisomerism deriving from a restricted rotation around a single bond, axially chiral compounds include spiranes, allenes, and alkylidenecycloalkanes. 26 Although asymmetric organocatalytic syntheses have been explored for the first two types, 27 the enantioselective synthesis of alkylidenecycloalkanes is underdeveloped. Bernardi reported the first asymmetric synthesis of axially chiral alkylidenes via an organocatalytic Wittig reaction (Scheme 17). 28 The [2 + 2] cycloaddition step between 4-substituted cyclohexanones 41 and stabilized phosphorus ylides 42 was catalyzed by TADDOL 43, which was able to break the symmetry plane of the substrate, thereby affording the final enantioenriched product 44 with low enantiocontrol.
With the aim of realizing enantioselective synthesis of axially chiral alkylidene scaffolds, we decided to exploit an asymmetric Knoevenagel olefination reaction. 29 The first asymmetric Knoevenagel condensation was realized by List, 30 who conducted the reaction between α-branched aldehydes and 1,3-dicarbonyl derivatives. Under the effective control of a cinchona primary amine catalyst, enantioenriched olefins were obtained through a dynamic kinetic resolution pathway.
Differently substituted oxindoles were tested, and important results were obtained. Highly insoluble substrates and oxindole bearing a strong electron-withdrawing group resulted in a decrease in yield and stereoselectivity (48c−f). Cyclohexanones with aromatic and aliphatic substituents produced excellent yield and demonstrated good-to-moderate enantiocontrol (48g−i). Interestingly, a cyclobutanone derivative could be used; however, the corresponding olefination product could be obtained in moderate yield and enantioselectivity (48j). A further enantioenrichment of the product was observed when the crude reaction mixture was filtered using a PTFE syringe filter. This outcome was caused by preferential precipitation of scalemic fractions of the products, thereby leaving the enriched major enantiomer in solution (ee values in brackets for 48a, 48c, 48f, 48h). The absolute configuration was assigned based on the TD-DFT calculations of the electronic circular dichroism spectra and set as R a . To elucidate aspects of the reaction mechanism, DFT calculations were performed in detail (Scheme 19).
The search of the TS resulted in locating two lowest energy structures corresponding to the selective attack of the Re face of the iminium ion from both faces of the oxindole (intermediates A and B, Scheme 19). Two possible elimination paths were considered: E2 and E1cb. The first path is stereospecific, and the final stereochemistry would depend on the geometry of the addition of TS1. The second path is stereodetermining, as two interchangeable diastereomeric rotamers of the enolate are formed (TS2) under the Curtin−Hammett profile. The ratio of the products depends on the energy difference between the E1cb TSs and is solely determined by the catalyst. All the corresponding E2/E1cb TSs were located at certain energy values, indicating that the R a product was preferred in both elimination routes. For Knoevenagel condensation, the E1cb

Scheme 18. Axially Enantioselective Knoevenagel Condensation
Accounts of Chemical Research pubs.acs.org/accounts Article path was considered, as it possessed low energy, and the calculated product ratio agreed well with the experimental value.
In this case, the catalyst played a fundamental role in controlling the stereochemistry of the final product.

DIRECT SYNTHESIS OF CONFORMATIONALLY RESTRICTED DIASTEREOISOMERS
Direct synthesis of C−C and C−heteroatom atropisomers has been a challenge, which is addressed by metal-catalyzed reactions. The use of coupling reactions provides the preferential route to synthesize atropisomeric compounds, particularly biphenyl and binaphthyl. 31 Over 15 years, organocatalysis has been able to face the aforementioned challenge, introducing an alternative method to classical desymmetrization or kinetic or dynamic kinetic resolution, which has been successfully applied to synthesize atropisomers. A venerable case has been realized by Jørgensen, who reported formation of β-hydrazino-naphthol atropisomers through the reaction between azodicarboxylates . 32 However, a step forward has been achieved when C−C atropisomers have been prepared via direct arylation of quinones. In 2015, Tan and Liu reported the enantioselective synthesis of biaryldiols catalyzed by chiral phosphoric acid, such as (S)-TRIP 65 (Scheme 21). 33 Excellent reactivity was achieved via catalyst-promoted conjugate addition of naphthols to quinones followed by aromatization of the resulting intermediate. The high enantioselectivity observed in the final biaryldiols 56a−g was achieved under the influence of the H-bonding network that assists the addition and aromatization steps. Other studies have highlighted the efficiency of different types of organocatalysts to realize analogous direct coupling reactions. 34 In addition to the studies conducted by Bella and Miller using a cinchona alkaloid and a short peptide as the catalyst, respectively, 35 the synthesis of C(sp 2 )−C(sp 3 ) atropisomers herein represents another exam-ple of direct synthesis of C−C atropisomers. This field, which has been rarely explored, is an interesting and challenging topic that draws inspiration from natural substances with complicated molecular architectures, such as cordypyridone A and B and pegaharmol A and B (Figure 2). Furthermore, C(sp 2 )−C(sp 3 ) conformational diastereoisomeric atropisomers were developed in the second half of the 1970s, when O̅ ki and Ford reported the isolation and rotational barrier determination of aryltriptycenes and 9-aryfluorenes, respectively. Dubois observed conformational isomerism in otolylcarbinols ( Figure 3). 36 Conformational diastereoisomers featuring a relatively slow barrier to rotation have been observed in sporadic cases and are considered effective intermediates for realizing synthesis of biaryl atropisomers through central-to-axial chirality conversion, as highlighted by studies conducted by Bertuzzi and Corti and Rodriguez and Bonne (Scheme 22). 37 We explored enantioselective preparation of C(sp 2 )−C(sp 3 ) conformational diastereoisomers, achieving thermodynamic control over the axial conformation of indanone derivatives by  Accounts of Chemical Research pubs.acs.org/accounts Article reacting substituted β-naphthols and inden-1-ones via iminium ion catalysis. 38 We established that β-naphthol and inden-1-one efficiently reacted and generated an enantioenriched mixture of chiral antiperiplanar (ap) and synperiplanar (sp) conformers, which were evident at the NMR spectroscopy time scale and room temperature (Scheme 23). 39 This observation led us to confirm that a slow rotation along the formed single bond was responsible for the conformational equilibrium observed. We effectively measured a rotational energy barrier of 17.8 kcal/mol considering the steric interaction between C4 of indanone and C8 of β-naphthols responsible for the relatively high rotational energy barrier. We studied this effect in detail, and by increasing the steric hindrance at C8 of naphthol and C4 of indanone, we attempted to obtain a thermodynamic preference for one conformer and rotationally stable C(sp 2 )−C(sp 3 ) atropisomers. Our concept was successfully addressed by using large substituents at ideal positions. A gradual increment in the rotational energy barrier, accompanied The entire process can be rationalized by understanding the role of the primary amine catalyst 46 and trifluoroacetic acid cocatalyst. We presume that nucleophilic addition of naphthol to the iminium ion is activated by the trifluoroacetate anion, and the resulting C(sp 3 )−C(sp 3 ) intermediate is harnessed in a closed rigid geometry that prevents it from free rotation along the new C−C bond. Rapid aromatization furnished the observed major ap-conformational diastereoisomer (Scheme 25).
The DFT calculation supports the existence of the sole ap conformer, as an energy barrier of >4 kcal/mol separates the ap from the sp ground states. The rotational energy barrier for interconversion of ap to sp conformer of compound 66 was 25.7 kcal/mol. This value suggests the existence of alkylated naphthols as axial diastereoisomers, in which a stable C(sp 2 )− C(sp 3 ) stereogenic axis connects the two reactive units.
The first enantioselective synthesis of the C(sp 2 )−C(sp 3 ) atropisomer was realized by Sparr, who performed efficient Rhcatalyzed [2 + 2 + 2] cyclotrimerization, controlling the formation of more than six stereoisomers of the sole aptriptycene derivative (Scheme 26a). 40 The enantio-and diastereoselective synthesis of atropisomeric cyclo[3.2.2]azines was realized by Jørgensen et al. (Scheme 26b). 41 The reaction represents a rare case of the kinetic control of the C(sp 2 )− C(sp 3 ) stereogenic axis, providing access to new atropisomeric cyclazine scaffolds.

ENANTIO-AND DIASTEREOSELECTIVE SYNTHESIS OF N−N ATROPISOMERS
The constant search for atropisomers that differ from the classical C−C or C−X framework, where X is O, S, and N, has  43 Moreover, application of 2,2′-bis(diphenylphosphino)-1,1′bibenzimidazole as ligands for asymmetric synthesis 44 and use of 9,9′-bicarbazole derivatives as functional materials 45 51 Owing to the importance of N−N atropisomers and our interest in the search for novel strategies for synthesis of atropisomers, we performed the first catalytic stereoselective synthesis of hydrazides containing a rotationally stable N−N single bond. 3 Our catalytic strategy was based on the use of azodicarboxylate derivatives as a precursor of the N−N single bond. At the design stage, we reasoned that azodicarboxylates can act as reagents in two catalytic reactions that sequentially work together to increase the steric hindrance around the N−N single bond, affording rotationally stable chiral tetrasubstituted hydrazides. To pursue our aim, we selected amination of racemic α-branched aldehydes 85 using 9-epi-9-amino-9-deoxyquinine 13 primary amine as the catalyst and the N-alkylation of the resulting trisubstituted hydrazide 86 using the commercially available quinidinium bromide salt 88 (Scheme 29).
The asymmetric synthesis of atropisomeric hydrazides performed using a one-pot protocol is applicable to a large series of branched aldehydes and benzyl bromides bearing different substituents (89a−l). This process furnishes a high level of enantioselectivity and diastereoselectivity and can be extended to different electrophiles, such as allyl iodide (89j) and Morita−Baylis−Hillman carbonate (89l) albeit with poor yields and diastereoselectivity. The N-benzylquinidinium bromide salt 88 exhibited good control of the stereogenic axis, revealing a preference for the R a configuration. The rotational energy barrier was experimentally determined as 28.3 kcal/mol for compound 89a; however, hydrazide intermediate 86 is presumably a transient atropisomeric molecule possessing a calculated rotational energy barrier of 20.6 kcal/mol. Interestingly, we investigated the possibility to realize stereodivergent synthesis that provides access to stereoisomers based on a simple catalyst permutation (Scheme 30). Enantiocontrol was well maintained, and diastereoselectivity was not considerably high owing to a matched/mismatched effect between the PT catalyst Accounts of Chemical Research pubs.acs.org/accounts Article combination and stereocenter of the tertiary hydrazide intermediate. When low enantiocontrol was observed in the amination reaction using catalyst 46, i.e., the quasi-enantiomer of catalyst 13 (96% ee vs 65% ee), diatsereoselectivity was low in the resulting tetrasubstituted hydrazide. The reaction mechanism has not been deeply investigated, particularly the atropselective alkylation step. However, we believe that the bifunctional nature of the PT catalyst 88 is fundamental for determining the stereogenic axis configuration observed. The hydroxide anion of I deprotonates the intermediate hydrazide, and the N-benzylquinidinium cation simultaneously interacts with the negatively charged nitrogen atom via ion pairing and with the carbonylic oxygen atoms via hydrogen bond-forming intermediate II. As shown in Scheme 31, the interactions preferentially occur on the hydrazide anion precursor of the final configuration because a compact associated species is formed.

CONCLUSION AND OUTLOOK
We have presented our findings on the synthesis of different classes of atropisomers focusing on the use of cinchona alkaloid derivatives as organic catalysts. The obtained results reinforce the strategic position occupied by organocatalysis in enantioselective preparation of C−N, C−C, and N−N axially chiral compounds. New enantioselective transformations that furnish high molecular complexity can be realized using accessible catalysts prepared from commercially available sources. In most transformations highlighted, the activity of the cinchona organocatalyst revealed is comparable with those of active metal species using covalent and noncovalent activation modes. This is also the case with olefination via the Knoevenagel reaction and Friedel−Craft-type alkylation of naphthols, in which addition to an iminium ion intermediate occurs efficiently using bulky reaction partners under mild reaction conditions. Furthermore, another important feature is the ability of cinchona organic catalysts, such as primary amines or organic bases, to efficiently differentiate the enantiotopic faces of proatropchiral substrates, exploiting their ability to realize fruitful secondary interaction (π-stacking, hydrogen bonds, ion pairing) or to be part of a strong catalytic cooperation with acidic cocatalysts, similar to the case of vinylogous desymmetrization of maleimides. We have focused on the development of novel atropselective reactions; it prompted us to explore new fields and molecular complexity as demonstrated by the recent results of the synthesis of N−N atropisomers, where an efficient onepot sequential relay catalysis was conducted using two different activation modes based on the use of a primary amine and PT catalyst. Although we have demonstrated that cinchona catalysts can be largely employed in the synthesis of molecules containing stereogenic axes and centers, the catalytic efficiency and versatility can be certainly improved. For these reasons, novel catalysts, enabling the use of cinchona alkaloid derivatives in industrial applications or to achieve a novel family of atropisomers, are highly required.